The first five main series games feature Desmond Miles, a direct descendant of their respective lead characters who are members of familial lines that had sworn an allegiance to the Assassins, as the modern-day protagonist. A convention established by the first game involves the player experiencing the lives of these characters as part of a simulation played by another in-game protagonist from the modern day era, using a technological device known as the Animus that is developed by Abstergo Industries, a corporate front of the Templar Order in the modern era. Most video games in the Assassin's Creed series tend to feature standalone or self-contained stories told within a fictionalized version of real-world historical civilizations, with at least one lead character from that setting and time period the vast majority of these characters are members of the Assassins or a related organization at one point in their lifetime.

This article describes major historical and fictional characters of the series that appear in the video games and live-action film adaptation. The series features original characters intertwined with real-world historical events and figures, and is centered on a fictional millennia-old struggle for peace between the Assassin Brotherhood, inspired by the real-life Order of Assassins, fight for free will, and embody the concept of chaos and the Templar Order, inspired by the real-life Knights Templar, desire peace through control, and embody the concept of order. The series also encompasses a wide variety of media outside of video games, including novels, comic books, board games, animated films, a live-action film, and an upcoming television series for Netflix. The Assassin's Creed media franchise, which primarily consists of a series of open-world action-adventure stealth video games published by Ubisoft, features an extensive cast of characters in its historical fiction and science fiction-based narratives.

From left to right: Adéwalé, Ratonhnhaké:ton, Arno Dorian, Altaïr Ibn-LaʼAhad, Evie Frye, Bayek, Kassandra, Alexios, Amunet, Jacob Frye, Ezio Auditore, Edward Kenway, Aveline de Grandpré, Shay Cormac. An illustration of some of the protagonist characters in the Assassin's Creed series.

Film assasin creed